Here’s an interesting nugget of information about how GPOs are applied in a SoftGrid/App-V environment. The short version is that in 4.2 and earlier, policies were applied to the VReg and now in 4.5 they’re not, but here’s the full explanation from Matt Young, a senior SDE on the App-V team: “Each Group Policy Object (GPO) is manifested in the registry, in the form of keys/values, and they typically live under { HKCU || HKLM }\Software\Policies.  These registry keys are pushed down from Active Directory upon user logon and in response to other events, as configured by the domain administrator.[…]”

More info: App-V blog